Pilot-scale　bio-filters　were　constructed　to　removal　of　high-iron,　manganese　and　ammonia　nitrogen　(8~10,　TFe　6~14mg/L,　Mn　0.8~1.5mg/L,　NH4+-N　2.0~3.0mg/L)　from　groundwater　and　the　oxidation　kinetics　was　analyzed.　The　results　showed　that　Aeration　and　Filter　process　and　Aeration　and　Double　Filtration　process　failed　due　to　lack　of　dissolved　oxygen　(DO).　Biological　Aerated　Filter　was　the　worst　process　due　to　aeration　on　the　water　flow　turbulence;　Biological　Aerated　Filter　and　Filtration　process　was　successful,　However,　the　filter　rate　was　only　6.0m/h;　Double　Aeration　and　Double　Filtration　(DADF)　was　the　best　process,　which　was　successful　to　removal　of　high-iron　manganese　and　ammonia　nitrogen　from　groundwater　(8~10,　TFe　17.66mg/L,　Mn　1.71mg/L,　NH4+-N　3.37mg/L),　and　the　maximum　filter　rate　of　the　first　stage　and　the　second　stage　were　13.25m/h　and　12.75m/h.　Moreover,　which　also　could　be　applied　to　the　purification　of　low-temperature　groundwater　(5~6,　TFe　9.72mg/L,　Mn　3.29mg/L,　NH4+-N　3.44mg/L),　and　the　maximum　filter　rate　of　the　first　stage　and　the　second　stage　were　10.0m/h　and　8.0m/h.　The　Oxidation　kinetics　demonstrated　that,　iron　was　oxidized　and　removed　followed　the　first-order　chemical　oxidation　kinetics,　and　the　oxidation　kinetics　constant　was　(1.02~1.18)×10,　and　the　removal　of　manganese　and　ammonia　nitrogen　followed　the　kinetic　law　of　zero-order　enzymatic　reaction,　and　the　oxidation　kinetics　constant　were　(0.15~0.83)×10-1　and　(0.31~1.20)×10-1.　The　oxidation　rate　of　iron　was　the　fastest　and　was　first　removed.　The　order　of　oxidation　and　removal　of　manganese　and　ammonia　nitrogen　was　greatly　affected　by　substrate　concentration.　©　2017,　Editorial　Board　of　China　Environmental　Science.　All　right　reserved.