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Glycogen accumulating organisms (GAOs) has been enriched in the anaerobic-aerobic sequencing batch reactor (AO-SBR) (drained after anaerobic) with glucose as the carbon source and the P/C ratio less than 2/100. PRA was less than 1.0 mg•L-1. The content of gly was 1.2 times the initial stage of GAOs enrichment. The domesticated GAOs cultured in anaerobic-anoxic operation could proceed endogenous denitrification reaction with NO2--N and NO3--N as endogenous carbon source. GAOs used intracellular poly-β-hydroxyvalerate (PHV), poly-β-hydroxyvalerate (PHB) and glycogen (gly) in turns as carbon source in endogenous denitrification process. The average endogenous denitrification rate of DGAOs using NO2--N and NO3--N as electron acceptors were 0.067 g N•(g VSS)-1•d-1 and 0.023 g N•(g VSS)-1•d-1 at 22, respectively. Short-range endogenous denitrification rate was about three times as much as endogenous denitrification rate at normal temperature. © All Right Reserved.
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