Lab-scale　completely　autotrophic　nitrogen　removal　over　nitrite　(CANON)　process　was　operated　in　Municipal　Waste　Water　Treatment　Plant　(WWTP).　The　tests　leading　to　two　started　strategies　of　CANON　filter,　R1was　filled　with　filler　with　matured　ANAMMOX　bacteria　and　inoculated　nitrite　flocculent　sludge,　R2　started　with　mixing　nitrification　filter　and　anaerobic　ammonium　oxidation　filter　with　weight　ratio　of　2:　1.　The　inlet　water　of　R1　and　R2　was　domestic　sewerage　after　the　A/O　phosphorus　removal　treatment　and　keeping　the　temperature　between　15~21degree　and　ammonia　nitrogen　concentration　within　35~55mg/L.　The　R1　and　R2　made　use　of　37d　and　19d　to　keep　ammonia　removal　rate　stable　at　95%.　After　operated　100d,　the　effluent　of　reactor　almost　did　not　contain　ammonia　and　the　maximum　effluent　total　nitrogen　concentration　of　R1and　R2　was　15.8mg/L　and　12.1mg/L　because　of　excessive　proliferation　of　NOB.　Specially　the　more　worse　of　R1,　and　effluent　of　R2　met　the　first　level　A　emission　standards.　The　start-up　strategy　of　mixed　media　reduced　two　thirds　of　the　amount　used　in　the　anaerobic　ammonium　oxidation　filter　media,　accelerated　the　start-up　of　the　reactor,　inhibited　the　activity　of　nitrite　oxidation　better,　and　achieved　the　long-term　nitrogen　emission　standards.　©　2017,　Editorial　Board　of　China　Environmental　Science.　All　right　reserved.