The　shaking　table　based　real-time　dynamic　substructuring　(RTDS)　technique　is　an　important　branch　of　modern　structural　testing　techniques,　which　can　simulate　the　dynamic　behaviors　of　soil-structure　interactions　and　vibration　absorber　systems　(e.g.　TLD　and　TMD)　experimentally　in　terms　of　a　large　specimen.　The　stability　of　RTDS　plays　a　key　role　in　the　implementation　of　a　test.　However,　the　complex　dynamics　of　a　shaking　table　causes　the　inaccuracy　of　RTDS　stability　prediction.　A　new　predication　method　was　developed　with　the　combination　of　root　locus　technique　and　the　modeled　comprehensive　dynamics　of　a　shaking　table.　The　feasibility　of　this　method　was　verified　experimentally.　Meanwhile,　the　drawback　of　the　commonly　used　method　was　analyzed　comparatively　through　phase　lag　and　magnitude　error.　Finally,　the　influence　of　structural　properties　on　RTDS　stability　was　discussed.　This　results　show　that,　with　the　consideration　of　the　integral　dynamics　of　a　shaking　table,　the　predication　accuracy　of　RTDS　stability　can　be　improved　greatly.　©　2016,　Engineering　Mechanics　Press.　All　right　reserved.