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Enriching mixed microbial culture (MMC) with capacity of PHA storage via controlling the enrichment process parameters is the first and the most important step for the fermentation production of PHA. This paper elucidated the role of sludge retention time (SRT) in the enrichment process under batch culture conditions. The results showed that SRT influenced the specific substrate degradation rate (qS), specific PHA accumulation rate (qP) and the PHA storage capacity of enriched MMC. The long SRT (SRT-4 d) led to the decreased qS, qP and PHA accumulation ability. The short SRT reduced the PHA conversion rate (YPHA/S) and PHA accumulation ability of MMC. The optimal SRT was determined as 2 d for enriched MMC in this study, which presented 56% cell dry weight PHA content. The study proved that the SRT played the important role in enriching halophilic MMC, which laid the foundation on enriching PHA-storage MMC rapidly. © All Right Reserved.
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