A　three-dimensional　seismic　isolation　bearing　(3DSIB)　was　introduced.　The　3DSIB　was　designed　and　applied　in　a　single-layer　spherical　lattice　shell　structure　with　40　m　span　by　using　a　SAP2000　software.　The　original　record　of　the　Taft　seismic　wave　was　input　into　the　shell　structure　in　X,　Y　and　Z　directions,　respectively　in　the　model.　The　effectiveness　of　the　3DSIB　was　examined　by　time　history　analysis　to　the　shell　structural　model　in　the　SAP2000.　It　was　shown　that　the　isolated　structure　with　3DSIBs　had　better　performance　compared　with　the　un-isolated　structure　in　terms　of　the　nodal　absolute　acceleration　response　as　well　as　member　axial　force.　It　was　found　that　the　3DSIB　had　good　isolation　function　in　both　horizontal　and　vertical　directions　for　shell　structure　under　seismic　conditions.　Furthermore,　the　influence　of　horizontal　and　vertical　stiffness　of　3DSIB　on　the　structural　period,　structural　member　peak　axial　force　and　nodal　absolute　acceleration　were　analysed.　The　variation　of　the　structural　responses　under　different　conditions　was　discussed.　The　results　will　provide　references　for　parameter　design　of　the　bearing　in　spatial　structures.　The　purpose　of　this　research　is　to　introduce　the　new　3DSIB　and　promote　the　practical　application　of　bearings　in　spatial　structures.　©　2016　The　Hong　Kong　Institution　of　Engineers.