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Nitrospira and Nitrobacter are two dominant types of nitrite-oxidizing bacteria (NOB).To evaluate the effect of dissolved oxygen (DO) on microbial community of NOB under the intermittent-aeration mode, the population and microbial community of this two NOB types were investigated in an aeration reactor with low DO period (55d) and high DO period (113d). Results showed the population of Nitrospira was much higher than that of Nitrobacter during the low DO period (0.2~0.5mg/L), and most clones of Nitrospira was very similar to the clone Candidatus Nitrospira defluvii. After increasing DO to a high level (1.5~2.3mg/L) and operating for 113d, Nitrobacter turned to the dominant group. Most clones of Nitrobacter were distributed to the branch containin Nitrobacter winogradskyi. Furthermore, the nitrite accumulation occurred in this reactor during the shift of population between Nitrospira and Nitrobacter after increasing DO, and it gradually disappeared when Nitrobacter became the dominant NOB group. © 2016, Editorial Board of China Environmental Science. All right reserved.
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