The　two-sludge　denitrifying　phosphorus　removal　system　(A2N2)　with　＇anaerobic/nitrification/anoxic/nitrification＇　operation　mode　was　used　to　treat　low　C/N　ratio　real　domestic　wastewater　in　sequencing　batch　reactors　(SBR).　Through　the　operating　condition　optimization　of　A2/O-SBR　and　N-SBR　unit,　the　system　achieved　efficient　denitrifying　phosphorus　removal　performance　with　the　low　carbon　source　sewage　and　solved　the　problem　of　high　NH4+-N　in　A2N-SBR　process.　Experimental　results　showed　that　post-aeration　was　essential　to　the　efficient　nutrient　removal　performance　and　long-term　stable　operation　of　the　A2N2-SBR.　Complete　denitrifying　phosphorus　uptake　without　aerobic　phosphorus　uptake　at　the　anoxic　stage　of　the　A2N2-SBR　was　disadvantageous　to　the　growth　and　reproduction　of　denitrifying　phosphorus　accumulating　organisms　(DPAOs).　Anaerobic　duration　of　1.5　h　in　the　A2/O-SBR　was　ensured　the　complete　oxidation　of　biodegradable　COD,　and　nitrification　duration　of　5　h　in　the　N-SBR　provided　sufficient　time　for　NH4+-N　oxidation.　With　anaerobic　phosphorus　release　of　1.5　h,　denitrifying　phosphorus　uptake　of　2　h,　and　aerobic　phosphorus　uptake　of　0.5　h　in　theA2/O-SBR　unit,　and　the　first　nitrification　of　5　h　and　second　nitrification　of　1h　in　the　N-SBR　unit,　the　average　effluent　COD,　NH4+N,　TN　and　PO43--P　concentrations　in　the　A2N2-SBR　system　was　46.2,　0.82,　13.63　and　0.82　mg/L,　respectively.　Simultaneous　nitrogen　and　phosphorus　removal　was　achieved　in　the　novel　A2N2-SBR　system.　©　2016,　Editorial　Board　of　China　Environmental　Science.　All　right　reserved.