• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
搜索

Author:

Wang, Yufeng (Wang, Yufeng.) | Zou, Mingqiang (Zou, Mingqiang.) | Chen, Yan (Chen, Yan.) | Tang, Fang (Tang, Fang.) | Dai, Jianjun (Dai, Jianjun.) | Jin, Yong (Jin, Yong.) | Wang, Cong (Wang, Cong.) | Xue, Feng (Xue, Feng.)

Indexed by:

EI Scopus SCIE

Abstract:

Sulfamethazine (SM2) is an antibacterial drug,which has been extensively used in human and veterinary medicine, long-term consumption of which may lead to the accumulation of sulfonamides in the body. Detection of sulfonamides often uses microbiological approaches, mass spectrometry and chromatography, which are expensive and time-consuming. Surface-enhanced Raman scattering-based immunochromatographic assay (SERS-ICA) has been recently applied in the detection. Herein, a Janus-labeled Au nanoparticle with subnanosized SiO2-monoclonal antibody and SERS reporter (DTNB) modified simultaneously (mAbAuNpDTNB) has been developed in a SERS-based lateral flow immunosensor, which can be used for rapid, quantitative and ultrasensitive detection of sulfamethazine residue in milk. The mAbAuNpDTNB exhibits a specific array on a paper stripe, which not only identifies sulfamethazine but also straightforwardly exposes the Raman reporter between the AuNps via self-assembly. The detection sensitivity of SERS-ICA for sulfamethazine reached 0.1 pg/mL, which was far below the previously published value by ELISA and the maximum residue limit set by the European Union. The entire SERS-ICA detection for sulfamethazine was completed within 15 min. Furthermore, high accuracy for this assay was exhibited in the spiking experiment with a recovery percentage of 88.1%-112.7%. The results demonstrated that this SERS-ICA can potentially be applied in point-of-care testing as an ultrasensitive and quantitative to semi-quantitative analytical method.

Keyword:

Immunochromatographic assay Janus Au nanoparticles Surface-enhanced Raman scattering Sulfamethazine

Author Community:

  • [ 1 ] [Wang, Yufeng]Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Peoples R China
  • [ 2 ] [Tang, Fang]Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Peoples R China
  • [ 3 ] [Dai, Jianjun]Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Peoples R China
  • [ 4 ] [Xue, Feng]Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Peoples R China
  • [ 5 ] [Zou, Mingqiang]Chinese Acad Inspection & Quarantine CAIQ, A 3,Gaobeidian Rd,Chaoyang Dist, Beijing 100123, Peoples R China
  • [ 6 ] [Chen, Yan]Chinese Acad Inspection & Quarantine CAIQ, A 3,Gaobeidian Rd,Chaoyang Dist, Beijing 100123, Peoples R China
  • [ 7 ] [Jin, Yong]Chinese Acad Inspection & Quarantine CAIQ, A 3,Gaobeidian Rd,Chaoyang Dist, Beijing 100123, Peoples R China
  • [ 8 ] [Xue, Feng]Nanjing Agr Univ, Sanya Inst, Sanya 572025, Peoples R China
  • [ 9 ] [Wang, Cong]Beijing Univ Technol, Fac Mat & Mfg, Beijing Key Lab Microstruct & Properties Solids, Beijing 100124, Peoples R China

Reprint Author's Address:

  • [Xue, Feng]Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Peoples R China;;[Jin, Yong]Chinese Acad Inspection & Quarantine CAIQ, A 3,Gaobeidian Rd,Chaoyang Dist, Beijing 100123, Peoples R China;;[Wang, Cong]Beijing Univ Technol, Fac Mat & Mfg, Beijing Key Lab Microstruct & Properties Solids, Beijing 100124, Peoples R China;;

Show more details

Related Keywords:

Source :

TALANTA

ISSN: 0039-9140

Year: 2023

Volume: 267

6 . 1 0 0

JCR@2022

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 14

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

Affiliated Colleges:

Online/Total:638/5318030
Address:BJUT Library(100 Pingleyuan,Chaoyang District,Beijing 100124, China Post Code:100124) Contact Us:010-67392185
Copyright:BJUT Library Technical Support:Beijing Aegean Software Co., Ltd.