This　study　investigated　the　impact　of　FNA-based　pretreatment　on　sludge　disintegration　and　waste　activated　sludge　anoxic　fermentation　and　denitrification　process.　The　results　showed　that　the　amount　and　rates　of　soluble　chemical　oxygen　demand　(SCOD)　production　increased　with　the　increasing　FNA　concentrations　(0,　0.68,　1.35　and　2.03　mgN/L)　during　FNA-based　pretreatment　process,　of　which　SCOD　production　rate　were　0.66,　1.70,　2.13　and　2.70　mg/(VSS·h),　respectively.　Furthermore,　the　percentages　of　biocidal　cells　accounting　for　the　total　cells　of　waste　activated　sludge　increased　from　41%　to　80%.　For　the　waste　activated　sludge　anoxic　fermentation　and　denitrification　system,　FNA-based　pretreatment　could　not　only　made　the　SCOD　production　and　sludge　reduction　performance　increase　by　49%　and　41%,　respectively,　but　also　an　increase　of　denitrifying　capacity　of　this　system　by　40%　was　achieved.　In　addition,　FNA-based　pretreatment　successfully　reduce　the　percentage　of　N2O　accounting　for　the　reduced　NO2-　by　58%.　©,　2015,　Chinese　Society　for　Environmental　Sciences.　All　right　reserved.