Immobilized　Anammox　granules　were　used　as　sequential　biocatalyst　addition　to　substitute　particulate　sludge　for　starting　up　Anammox　reactor,　and　the　kinetic　characteristics　of　the　immobilized　granules　were　investigated　through　batch　tests.　The　reactor　could　be　successfully　started　up　in　49　d　with　immobilized　Anammox　granules　as　sequential　biocatalyst　addition,　and　the　removal　rates　of　NH4+-N　and　NO2--N　were　80.7%　and　83.1%,　respectively.　Kinetics　study　showed　that　the　half　saturation　constants　for　ammonium　and　nitrite　of　the　immobilized　granules　were　1.57　mmolL-1　and　1.505　mmolL-1,　the　half　inhibition　constants　of　ammonium　and　nitrite　were　724.2　mmolL-1　and　66.65　mmolL-1,　respectively.　The　immobilized　granules　were　easier　to　gain　substrate　in　adverse　environment　and　could　suffer　higher　substrate　concentration.　The　better　kinetic　performance　of　the　immobilized　Anammox　granules　contributed　to　the　successful　biomass　supplementation　performance,　which　was　helpful　for　the　novel　biomass　supplementation　process　for　enhancement　of　Anammox　performance.　©,　2015,　Chemical　Industry　Press.　All　right　reserved.