In　order　to　study　the　effect　of　adding　method　on　the　waste　activated　sludge(WAS)　alkaline　fermentation　in　the　low　temperature　(15±2).　The　WAS　were　fermented　in　the　NaOH,　KOH,　Ca(OH)2　and　mixed　alkalis　fermentation　systems　(pH=10±0.2),　and　changed　the　dosing　method　of　WAS　after　the　fermentation　system　stability(a　dosing,　average　two　dosing,　average　three　dosing).　Dissolution　of　organic　matters,　short　volatile　fatty　acids　(SCFAs),　soluble　protein,　soluble　polysaccharide　and　key　enzyme　(hydrolase　and　coenzyme　420)　were　analyzed　during　sludge　anaerobic　fermentation　process.　The　acetic　acid　in　the　SCFAs　was　also　observed.　The　batch-mode　results　showed　that　the　dosing　method　had　a　significant　impact　on　performance　of　the　hydrolysis　and　acidification.　The　soluble　chemical　oxygen　demand　(SCOD)　decreased　with　WAS　dosing　times,　where　soluble　protein　and　polysaccharide　increased.　The　hydrolytic　enzyme　decreased　with　WAS　dosing　times.　However,　the　coenzyme420　increased　in　the　NaOH　and　KOH　conditions,　remained　about　the　same　in　the　mixed　alkali　condition,　declined　in　the　Ca(OH)2　condition.　The　activity　of　acidification　declined　with　WAS　dosing　times　in　NaOH　and　KOH　conditions,　but　the　SCFAs　increased　in　average　two　dosing　and　decreased　in　the　average　three　dosing　in　the　Ca(OH)2　condition.　Acidification　performance　was　stable　relatively　in　the　Ca(OH)2　condition　and　the　proportion　of　acetic　acid　in　SCFAs　was　the　optimal　which　was　higher　10%　comparing　with　other　conditions.　©,　2015,　Chinese　Society　for　Environmental　Sciences.　All　right　reserved.