Modern　chromatography　is　increasingly　focused　on　miniaturization　and　integration.　Compared　to　conventional　liquid　chromatography,　microfluidic　chip　liquid　chromatography　(microchip　-LC)　has　the　potential　due　to　its　zero　-dead　volume　connection　and　ease　of　integration.　Nano　-sized　packings　have　the　potential　to　significantly　enhance　separation　performance　in　microchip　-LC.　However,　their　application　has　been　hindered　by　packing　difficulties.　This　study　presents　a　method　for　packing　nano　-sized　silica　particles　into　a　microchannel　as　the　stationary　phase.　The　microchip　-LC　packed　column　was　prepared　by　combining　the　weir　and　the　porous　silica　single　-particle　as　frit　to　retain　the　packing　particles.　A　surface　tensionbased　single　-particle　picking　technique　was　established　to　insert　porous　single　-particle　frit　into　glass　microchannels.　Additionally,　we　developed　a　slurry　packing　method　that　utilizes　air　pressure　to　inject　nano　-sized　packing　into　the　microchannel.　Pressure　-driven　chromatographic　separation　was　performed　using　this　nano　-packed　column　integrated　into　a　glass　microchip.　The　mixture　of　four　PAHs　was　successfully　separated　within　just　8　min　using　a　5　mm　separation　channel　length,　achieving　high　theoretical　plates　(106　plates/m).　Overall,　these　findings　demonstrate　the　potential　of　utilizing　nano　-sized　packings　for　enhancing　chromatographic　performance　in　microchip　systems.　(c)　2024　Published　by　Elsevier　B.V.　on　behalf　of　Chinese　Chemical　Society　and　Institute　of　Materia　Medica,　Chinese　Academy　of　Medical　Sciences.