The　platinum　loading　is　randomly　distributed　within　catalyst　layers　because　of　the　fabrication　way,　whereas　the　platinum　loading　is　considered　to　follow　the　homogeneous　distribution　in　the　traditional　catalyst　layer　model.　The　cell　performance　and　heat　and　species　transport　laws　of　the　fuel　cell　with　platinum　loading　random　distribution　within　catalyst　layers　are　not　yet　clear.　Therefore,　in　this　work,　a　two-dimensional,　non-isothermal　proton　exchange　membrane　fuel　cell　model　considering　the　two-phase　flow　and　agglomerate　structure　of　catalyst　layers　is　established　to　simulate　the　fuel　cell　having　the　catalyst　layer　where　platinum　loading　is　random　distribution　in　a　way　that　is　closer　to　the　actual　structure.　The　effect　of　random　distribution　of　platinum　loading　on　the　electrical　property,　species　transfer　and　temperature　distribution　is　analyzed.　Results　suggest　that　the　randomness　influence　needs　to　be　considered　when　the　local　distributions　of　parameters　are　studied　and　the　research　accuracy　of　parameters　is　relatively　high.　In　contrast　with　the　homogeneous　distribution　of　platinum　loading,　the　cell　performance　for　the　normal　random　distribution　and　uniform　random　distribution　cases　is　reduced.　The　effects　of　catalyst　loading　random　patterns　on　cell　performance,　species　and　temperature　distributions　are　different,　and　the　decrease　of　cell　performance　for　the　uniform　random　distribution　case　is　more　obvious.　Furthermore,　the　influence　degree　of　randomness　on　each　parameter　is　different.　The　distributions　of　electrochemical　reaction　rate,　oxygen　concentration　and　temperature　display　random　characteristics,　while　the　liquid　water　saturation　distribution　is　less　influenced　by　randomness　and　does　not　show　an　obvious　random　change.　These　findings　can　provide　a　reference　for　the　randomness　verification　in　random　simulations　and　deepen　the　understanding　of　species　and　heat　transfer　laws　in　the　catalyst　layer　which　is　closer　to　the　reality　to　improve　the　accuracy　of　fuel　cell　simulations.