Sucralose　(SUC)　is　an　artificial　sweetener　for　everyday　consumption　and　often　co　-exists　with　disinfectants　such　as　triclosan　(TCS)　and　dialkyldimethyl　ammonium　compound　(DAC)　in　sewage.　Considering　their　continuous　accumulation　in　sewage,　it　is　essential　to　understand　the　impacts　of　their　single　and　combined　stress　on　the　evolution　of　resistance　genes　(RGs)　and　microorganisms.　In　this　study,　three　sequencing　batch　reactors　(SBRs)　were　established,　which　were　added　with　SUC,　disinfectant,　SUC　and　disinfectant,　respectively,　and　named　SSBR,　DSBR　and　SDSBR　in　turn.　There　were　four　stages　in　total,　the　first　two　stages　and　the　latter　two　stages　were　set　to　explore　the　effects　of　co　-exposure　of　SUC　and　single　disinfectant　(TCS),　SUC　and　combined　disinfectants　(TCS　and　DAC)　on　nitrification　system,　respectively.　SSBR　showed　excellent　ammonia　oxidation　performance　in　the　whole　operation　stages.　Compared　with　DSBR,　the　microorganisms　in　SDSBR　under　the　combined　stress　of　SUC　and　TCS　were　less　inhibited.　TCS　and　DAC　destroyed　the　ammonia　oxidation　performance　of　DSBR　and　SDSBR.　Within　120　days,　the　removal　efficiency　of　TCS　reached　90　%.　In　SSBR,　1　mg/L　SUC　promoted　the　proliferation　of　RGs,　especially　induced　free　RGs　in　water　(w-RGs)　to　maintain　high　abundance　and　persistence.　Compared　with　single　stress,　the　abundances　of　intracellular　RGs　in　sludge　were　higher　under　the　combined　stress　of　SUC　and　TCS,　and　the　risk　of　RGs　transmission　was　greater.　The　combined　stress　of　SUC　and　disinfectants　(TCS　and　DAC)　led　to　a　higher　enrichment　of　w-RGs,　exacerbating　the　risk　of　w-RGs　transmission.