Molecular　catalysts　have　attracted　significant　attention　because　of　their　high　activity,　selectivity,　and　tunability.　However,　in　heterogeneous　catalysis,　the　uniform　dispersion　and　immobilization　of　molecular　catalysts　on　the　supporting　substrate　remain　a　significant　challenge　due　to　their　aggregation　tendency.　Here,　we　present　a　facile　strategy　to　molecularly　disperse　and　immobilize　a　series　of　macrocyclic　metal　complexes　onto　reduced　graphene　oxide　(rGO)　by　using　DNA　as　a　mediator.　The　electroactive　amounts　of　molecularly　dispersed　iron　phthalocyanine　(FePc)　molecules　are　increased　by　similar　to　50　times　greater　than　that　of　pristine　FePc　catalyst.　As　a　result,　the　single-molecule　catalyst　demonstrates　a　notable　power　density　(similar　to　290　mW　cm(-2))　in　an　H-2/O-2　alkaline　polymer　electrolyte　fuel　cell.　Operando　X-ray　absorption　spectroscopy　experiments　combined　with　density　functional　theory　calculations　reveal　that　the　coordination　interaction　between　FePc　and　DNA　enables　the　molecular　dispersion　and　immobilization　of　FePc　on　the　surface　of　rGO,　and　consequently　improves　the　activity　by　regulating　the　electronic　structure　of　active　centers.　This　study　points　out　a　facile　strategy　to　tackle　the　fundamental　challenges　facing　molecular　catalysts　in　long-lasting　energy　conversion　technologies.