Mesoporous　SiO2　was　coated　on　the　home-made　β-FeOOH　hollow　microspheres　from　simultaneous　polymerization　of　tetraethoxysilane　(TEOS)　in　presence　of　a　cationic　surfactant　(cetyltrimethylammonium　bromide;　CTAB)　and　two　swelling　agents,　decane　and　1,　3,　5-trimethylbenzene　(TMB).　The　mesoporous　SiO2/Fe3O4　hollow　microspheres　were　prepared　after　calcination　and　then　reducuction.　In　the　experiments,　two　swelling　agents,　decane　and　TMB　were　added　respectively　or　successively　to　prepare　microspheres　with　different　pore　sizes.　Furthermore,　the　hollow　microspheres　were　used　as　a　carrier　to　immobilize　laccase.　The　results　reveal　that　compared　with　that　of　a　single　swelling　agent,　the　mesoporous　SiO2/Fe3O4　hollow　microspheres　obtained　by　adding　two　swelling　agents　have　better　mesoporous　properties.　When　the　total　pore　swelling　agents　(CTAB)　molar　ratio　is　2　and　TMB/CTAB　molar　ratio　is　1,　the　mesoporous　SiO2/Fe3O4　hollow　microspheres　exhibit　a　high　surface　area　(855　m2·g-1),　large　average　pore　(5.72　nm),　high　pore　volume　(1.23　cm3·g-1).　The　hollow　microspheres　have　larger　adsorption　amount　and　adsorption　rate,　and　the　adsorption　amount　of　laccase　are　up　to　276　mg/g.　The　activity　of　the　immobilized　laccase　has　a　better　pH　and　thermal　stability　in　comparision　with　that　of　free　laccase.　The　mesoporous　SiO2/Fe3O4　hollow　microspheres　can　be　separated　in　an　applied　magnetic　field　rapidly.