Separation　of　monovalent　salt　and　antibiotics　is　of　vital　importance　in　membrane　field.　In　this　work,　zwitterionic　polyamide　membrane　was　prepared　by　incorporating　N,　N-diethylethylenediamine　(DEEDA)　into　interfacial　polymerization　firstly.　Then,　zwitterions　were　introduced　into　membrane　matrix　via　grafting　reaction　with　1,　3-propanesultone　(1,　3-PS).　The　resulting　zwitterionic　membrane　exhibited　optimal　permeability　of　14.6　Lm(-2)　h(-1).bar(-1)　and　high　rejection　of　organics.　Meanwhile,　owing　to　the　incorporation　of　zwitterions,　the　zwitterionic　membrane　showed　lower　rejection　of　monovalent　salt　compared　with　that　of　the　pristine　polyamide　membrane.　Thus,　the　zwitterionic　membrane　behaved　efficient　separation　ability　of　monovalent　salt/antibiotics　when　NaCl/erythromycin　mixture　solution　was　chosen　as　the　experimental　solution.　When　bovine　serum　albumin　was　used　as　a　foulant,　the　zwitterionic　membrane　also　exhibited　anti-fouling　property.　This　work　provided　a　facile　way　to　prepare　zwitterionic　membrane　with　efficient　separation　ability　of　monovalent　salt/antibiotics,　which　promoted　the　development　of　zwitterions　in　applications　of　nanofiltration　membrane　field.