The　dynamic　characteristics　of　joint　interfaces　have　significant　effect　on　both　static　and　dynamic　behaviors　of　the　whole　machine　tool　structures.　Its　dynamic　model　can　be　simplified　as　a　group　of　equivalent　spring-damping　elements,　that　is,　an　＇elastic　interlayer＇　without　mass.　The　unit　area　dynamic　characteristic　parameters　are　the　key　to　analyze　the　dynamic　properties　of　joint　interfaces.　However,　when　building　the　stiffness　and　damping　matrices　of　the　joint　interfaces,　the　interaction　between　the　＇elastic　interlayer＇　and　the　conjunctions　are　always　ignored,　which　leads　to　errors　arising.　A　test　system　for　identifying　the　unit　area　dynamic　characteristic　parameters　of　different　kinds　of　joint　interfaces　are　represented　based　on　Equivalent　Single　Degree　Of　Freedom　(ESDOF)　system　theory.　The　unit　area　dynamic　characteristic　parameters　can　be　applied　under　all　kinds　of　conditions　(including　different　materials,　pre-tightening　force,　surface　roughness,　lubricating　conditions,　media,　etc).　Then　a　comprehensive　unit　stiffness　matrix　is　derived　from　these　parameters.　The　presented　method　is　compared　with　the　conventional　method　on　analyzing　the　vibration　behavior　of　an　assembled　beam　structure.　The　comparison　results　show　that　the　presented　method　is　in　excellent　agreement　on　the　actual　conditions,　which　has　an　obvious　advantage　on　accuracy.　The　presented　method　can　be　used　as　an　effective　way　for　precisely　analyzing　vibration　behavior　of　complicated　mechanical　structures　with　plate-like　joint　interfaces.　©　2014　IFSA　Publishing,　S.　L.