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Effect of raw water quality on nitrification formation and its influence on effluent in water distribution pipes were studied by pipeline reactors simulated the water situation. Water samples were prepared in laboratory to simulate Class II, Class III and Class V of the surface water standard. The results showed that the concentration of ammonia nitrogen (NH4+-N) and dissolved oxygen (DO) in raw water had significant effect on NH4+-N removal. Under sufficient DO, removal rate of NH4+-N improved with the increasing NH4+-N concentration in raw water. When DO was insufficient, NH4+-N removal was limited by DO. Accumulation of nitrite nitrogen (NO2--N) was significantly affected by concentration of NH4+-N in raw water at initial stage of biofilm formation. NO2--N accumulated more serious with higher NH4+-N content, but the influence gradually weakened as the biofilm matured. The quantity of AOB was considerably affected by concentration of NH4+-N in raw water, and AOB quantity increased with the concentration of NH4+-N. The quantity of NOB was considerably affected by the concentration of both NH4+-N and DO in raw water. Low DO concentration would inhibit activity of NOB, resulted in fewer quantity of NOB and less accumulation of NO2--N. Nitrification in water distribution pipes was generated by nitrifying bacteria in the bulk water and biofilm, but biofilm nitrify bacteria had a higher level than bulk nitrify bacteria.
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