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The feasibility of completely autotrophic nitrogen removal process (CANON) for treating domestic sewage at ambient temperature was investigated in MBR. Firstly, the hydraulic retention time (HRT) was gradually reduced without variation of influent ammonia for enriching AOB, then HRT was further decreased with the reduction of aeration rate for enriching anammox, and DGGE was adopted in this study for analysis of the community dynamics in different phases. CANON was successfully started-up within 78 days, finally total nitrogen removal rate reached 0.96 kg·m-3·d-1 with the removal efficiency of 80%, COD and ammonia were simultaneously removed when treating domestic sewage. Phylogenetic analysis indicated that Nitrosomonas related ammonia oxidizing bacteria and Candidatus Kuenenia stuttgariensis like ANAMMOX bacteria existed in the reactor. The MBR-CANON system was feasible for treating domestic wastewater at ambient temperature.
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