The　startup　of　ANAMMOX　coupling　heterotrophic　denitrifying　reactor　and　the　influence　of　concentration　of　phenol　on　the　nitrogen　removal　performance　of　coupled　reactor　were　investigated.　Cultivated　with　2L　(20%　of　effective　volume　of　reactor)　anaerobic　ammonia　oxidation　granular　sludge　that　the　volatile　suspended　solids　(MLVSS)　was　6000　mg/L,　anaerobic　ammonia　oxidation　coupling　heterotrophic　denitrifying　was　started　up　successfully　for　86　days　under　the　condition　of　pH　7.8,　water　temperature　25°C　and　hydraulic　detention　time　(HRT)　1.5　h.　The　steady　phase,　the　average　removal　rates　of　NH4+-N,　NO2--N　and　TN　were　85.39%,　86.12%　and　79.85%　respectively,　the　average　volumetric　load　of　total　nitrogen　and　the　average　removal　load　of　total　nitrogen　were　2.63　kg/(m3·d)　and　2.10　kg/(m3·d)　respectively.　ANAMMOX　granular　sludge　was　coated　with　phenol　denitrifying　bacteria.　The　relationship　between　heterotrophic　denitrification　and　anammox　was　cooperative　and　competitive　in　the　system.　The　activity　of　ANAMMOX　bacteria　was　suppressed　strongly　when　the　concentration　of　phenol≥0.3　mmol/L,　the　competition　between　phenol　denitrifying　bacteria　and　ANAMMOX　bacteria　was　intensified　as　the　increasing　of　concentration　of　phenol.　When　the　concentration　of　phenol　was　0.2　mmol/L,　the　denitrification　effect　of　system　is　best　considering　from　denitrification　and　the　stability　of　system.　The　ratio　of　removed　NH4+-N,　reomved　NO2--N　and　generated　NO3--N　was　1:1.52:0.11.