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To investigate the fast start-up of CANON process and characterization of functional microbes, conventional activated sludge was inoculated to an MBR and the reactor was operated intermediately at ambient temperature. In the launch strategy, the regulation of aeration and aeration time was used as the main method. First, partial nitrification was applied under oxygen-limited condition. Secondly, DO had to be decreased further to achieve CANON process. Finally, aeration time and inorganic carbon concentration needed to be adjusted to improve the total nitrogen removal rate. Besides, the characterization of functional microbes in stable CANON process was analyzed using PCR-DGGE techniques. The results showed that the CANON process launched successfully after 36 days, the ammonium removal rate and total nitrogen removal rate were kept at around 99% and 84% and the maxinum nitrogen removal rate can reach 0.41kg/(m3·d). DGGE profiles showed Nitrosomonas and Candidatus Kuenenia stuttgartiensis were predominant microbes in the reactor and they worked synergetically to form an efficient autotrophic nitrogen removal process within the MBR. ©, 2014, Chinese Society for Environmental Sciences. All right reserved.
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