Glycosylation　is　a　common　form　of　protein　post-translational　modification　and　plays　key　roles　in　biological　processes,　which　is　closely　related　to　the　occurrence,　development,　diagnosis　and　treatment　of　various　diseases.　Although　the　rapid　development　of　biological　mass　spectrometry-based　technology　offers　great　conveniences　to　identification　of　glycosylation　sites,　peptides　sequences　and　glycan　structures　of　glycoproteins　respectively,　the　illumination　of　the　linkage　between　proteins　and　glycans　and　the　analysis　of　the　structure　of　intact　glycopeptides　still　face　a　lot　of　difficulties　and　challenges.　Based　on　literature　researches,　the　major　directions　of　the　field　were　determined.　This　review　will　focus　on　MS　analysis　of　the　site-specific　intact　glycopeptides　and　describes　the　development　and　prospects　of　biological　mass　spectrometry-based　technology　for　the　analysis　of　intact　glycopeptides　on　many　aspects,　from　ion　source　and　mass　analyzers　to　dissociation　modes　and　the　analysis　of　mass　spectrometric　data.