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The pre-denitrification SBR process of treating municipal waste water including phenol was investigated with the combination of short-term and long-term tests. The results show that the ammonia oxidation rate decreases gradually with the increase of concentration of phenol (0-175 mg/L), with the specific substrate ulitization rates ranging of 2.898-0.694 d-1. The average ammonia oxidation rate is 4.091 mg/(g·h) in pre-denitrification system and 2.26 times short-term test result (1.812 mg/(g·h)). The ratio of ammonia oxidation rate to phenol concerntration keeps at -0.031±0.005. The phenol and ammonia can be removed at 0-5 h with the respective removal efficiencies of 24.2% and 23.5%. Moreover, the ammonia oxidation damage can be recovered to normal levels after 15-18 d through microbial own structure function change. However, the toxicity inhibition at the high phenol concentrations exhibites an irreversible damage, and DNA concentration in extracellular polymer substance varies from 2.53 mg/g up to 34.6 mg/g.
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