To　study　the　long　term　effect　of　glucose　on　the　performance　of　enhanced　biological　phosphorus　removal　(EBPR)　system,　a　laboratory　scale　sequencing　batch　reactor　(SBR)　fed　with　glucose　was　operated　for　364　days.　The　SBR　performance　of　three　typical　cycles　in　different　periods　was　compared.　The　effect　of　glucose　on　anaerobic　substrate　storage　and　conversion　of　phosphate-accumulating　organisms　(PAOs)　was　discussed,　and　the　quantity　of　populations　of　PAOs　and　glycogen　accumulating　organism　(GAOs)　was　detected　with　fluorescent　in　situ　hybridization　(FISH).　The　results　indicate　that　the　system　shows　high　and　stable　phosphorus　removal　efficiency　after　180　days,　the　highest　phosphorus　release　can　reach　80　mg/L　and　the　effluent　concentration　of　PO43--P　is　less　than　1　mg/L.　Compared　with　the　typical　cycles　in　the　1st　and　2nd　period,　the　typical　cycle　in　the　3rd　period　showed　the　best　performance,　the　anaerobic　phosphorus　release　reaches　79　mg/L　and　phosphorus　release　rate　is　97.2%.　It　is　indicated　that　fermentative　bacteria　first　converts　glucose　to　volatile　fatty　acids　(VFAs)　and　then　PAOs　utilize　the　VFAs　to　synthesize　poly-hydro　xyalkanoate　(PHA).　The　proportion　of　PAOs　keeps　increasing　rapidly　and　reaches　45.5%　at　340　days.　The　FISH　analysis　results　indicate　that　it　is　possible　for　EBPR　using　glucose　as　the　sole　carbon　source　to　develop　a　system　with　high　proportions　of　PAOs.