Consortiums　from　anammox,　methanol　denitrification　process,　and　domestic　wastewater　treatment　plant　were　used　for　nitrogen　removal　in　a　multi-substrate　system　by　batch　tests.　The　activity　and　inhibition　characteristics　of　bacteria　in　denitrification　were　studied　seriously.　Experimental　results　showed　that　alcohols　inhibited　anaerobic　ammonium　oxidation　bacterium,　and　the　most　serious　inhibition　came　from　methanol,　leading　to　activity　lose　about　2/3　at　concentrations　of　5.48　mmol/L.　Sodium　acetate　strengthened　AAOB　activity,　meanwhile,　glucose,　lactose　and　sucrose　had　no　effect　on　its　activity.　These　experimental　results　clearly　indicated　that　the　best　hetero-denitrification　carbon　sources　for　methanol　denitrifiers　and　activity　sludge　were　methanol　and　sodium　acetate　respectively.　In　mixture　consortium,　inhibitor　propanol　made　the　AAOB　at　a　disadvantage　in　the　competition　with　the　methanol　denitrifiers,　on　the　other　side,　the　effect　from　sodium　acetate　to　AAOB　can　be　ignored　in　the　competition　with　activity　sludge.