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At room temperature, by Changing the substrate concentration of inflow, the biological nitrogen removal of up-flowing ANAMMOX biofilter loading volcanic rock filter at different ammonium concentrations was investigated. techniques of microscope and scanning electron microscopy (SEM) were utilized to analyze microorganisms form, denaturing gradient gel electrophoresis (DGGE), cloning and sequcening were used to identify species of ANAMMOX. The results of operation revealed that, at room temperature, the up-flow ANAMMOX biofilter had the ability to maintain high capacity of biological nitrogen removal under high and low substrate concentrations. The maximum total nitrogen removal load was up to 2.99kgN/(m3·d), and the maximum removal rate of NH4+-N and NO2--N was 99.4% and 98.8% respectively. Microscope and SEM show that the species of microorganisms is much richer at the bottom of biofilter, however, the proportion of ANAMMOX is much higher in the middle and upper of biofilter. The sequence of ANAMMOX 16SrRNA identification revealed that Candidatus Kuenenia stuttgartiensis occurred in the up-flowing ANAMMOX biofilter, which had wide adaptability to different temperature and substrate concentrations.
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