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The landfill leachate treatment was investigated by using an UASB-SBR biological system. The long-term stability of nitrogen removal via nitrite at normal and low temperature was studied. Furthermore, the achievement mechanism of partial nitrification and characteristic of nitrifying bacteria were investigated in SBR. 598days experimental result showed that the removal efficiencies of COD, NH4+-N and TN reached 92.0%, 99.2% and 98.0%, respectively. The advanced nitrogen removal was achieved in the system. Partial nitrification was achieved after 116days operation in SBR, and nitrite accumulation ratio (NAR) reached above 90%. The higher NAR was maintained for 171days at low 15°C and the lowest temperature of 10.2°C during two winters. The effect of free ammonia (FA) inhibition and process control was used to achieve nitrite pathway in the SBR. The ammonia oxidizing bacteria (AOB) was dominant by fluorescence in situ hybridization (FISH) techniques analysis, and the spherical Nitrosococcus and rod-shaped Nitrosomonas were dominant in ammonia oxidizing bacteria by scanning electron microscope (SEM) techniques analysis.
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