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The effect of DO (Dissolved oxygen) on the kinetics of nitrite denitrification, as well as the corresponding N2O accumulation and emission were investigated using 2.4 L sequencing batch reactor. The results show that NaNO2 is added at the initial of the test to the final NO2--N mass concentration of 40 mg/L. The aeration rate is controlled at 0.3 L/min and the ratio of N2 to O2 is adjusted so that the DO can be controlled at 0, 0.1, 0.3, 0.5 and 0.7 mg/L and the N2O emission is 0.41, 0.60, 2.62, 4.98, 6.83 mg/L, respectively. The denitrification rate decreases with the increase of DO mass concentration. The nitrite reduction rate per gram of mixed liquor suspended solid (MLSS) decreases from 14.9 to 10.2 mg/(L · h), while the N2O emission rate per gram of MLSS increases from 0.2 to 1.9 mg/ (L · h). The reason may be that higher DO mass concentration inhibits the activity of N2O reductase thus results in higher N2O production as to higher N2O emission. Besides, higher nitrite presence inhibits the activity of nitrous oxide reduction enzymes which is responsible for N2O reduction. To reduce DO mass concentration in anoxic tanks during nitrogen removal process may be the efficient way to reduce N2O emission during nitrite denitrification process.
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