Two　SBRs　are　first　used　to　train　activated　sludge　of　heterotrophic　denitrification　bacteria　for　nitrate　and　nitrite,　respectively.　Then,　the　activated　sludge　that　has　ANAMMOX　function　is　simultaneously　inoculated　into　the　two　reactors.　The　efficiency　of　the　coupling　activated　sludge　including　heterotrophic　denitrification　bacteria　and　ANAMMOX　bacteria　is　researched.　Experimental　results　show　that　the　efficiency　of　heterotrophic　denitrification　of　nitrite　is　better　than　that　of　nitrate.　However,　the　coupling　effect　with　ANAMMOX　bacteria　is　on　the　contrary.　Under　the　condition　of　aeration,　the　two　reactors　have　better　nitrification　function　at　the　same　time,　and　the　accumulation　ratios　of　nitrite　are　62.2%　and　87.3%,　respectively.