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A novel aerobic granular sludge (AGS) system called SBR (sequencing batch reactor)-CF (continuous-flow) system merging the advantages of sequencing batch reactors (SBRs) and continuous flow (CF) reactors was developed. The AGS was successfully operated in the SBR-CF system which consisted of four same SBRs (each served as settling tank/anaerobic feeding tank/aerobic reacting tank in turn). The effects of aeration intensity and hydraulic retention time (HRT) on the SBR-CF system were studied. The results showed strong aeration intensity (9.74 h(-1) in this study) and appropriate HRT (9 h in this study) were more favorable to the nutrients removal. The EEM-PARAFAC analysis was applied to characterize the LB-EPS, TB-EPS and domestic wastewater, as results TB-EPS was found play an important role in the biosorption in COD removal of the SBR-CF system. In addition, a preliminary conceptual reaction process model in the SBR-CF system was built using high-throughput pyrosequencing and phylogenetic assignment.
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