We　present　an　effective　and　low-cost　method　for　fabricating　microfluidic　chip　based　on　excimer　laser　direct-writing　ablation　and　replica　molding.　It　is　based　on　a　newly　excimer　laser　micromachining　technique　that　can　accurately　machine　microfluidic　chip　microstructure　with　smooth　surface　profile.　Microfluidic　chips　with　precise　and　smooth　surface　profiles　are　ablated　by　direct　laser　machining　on　an　epoxy　glue　layer　sticking　on　glass　substrates.　Laser-machined　microfluidic　chips　are　replicated　by　electroforming　to　obtain　inverse　metal　molds.　Finally,　polycarbonate　(PC)　microfluidic　chips　are　replicated　from　these　metal　molds　using　injection　molding　method.　The　relation　between　the　process　parameters　(the　translational　speed　of　working　platform　and　the　laser　fluence)　and　the　micromachining　quality　(the　depth　and　surface　of　the　microchannel)　is　investigated.　The　profile　accuracy　and　surface　roughness　of　the　produced　microfluidic　chip　at　each　stage　are　measured　and　monitored.　The　average　upper　surface　profile　accuracy　is　better　than　2　μm　and　the　average　surface　roughness　is　less　than　20　nm.　Experimental　data　show　the　controllability　and　accuracy　of　this　micromachining　process.　Experimental　investigation　is　performed　on　the　flow　characteristics　of　water　in　two　different　roughness　rectangle　microchannels.　The　flow　characteristics　of　water　in　the　microchannels　fabricated　with　the　proposed　combination　method　are　better　than　those　in　the　microchannels　fabricated　with　laser　direct-writing　when　the　flow　velocity　is　small.