To　analyze　the　effects　of　parameters　and　working　fluids　on　the　power　generation　using　LNG　cold　energy,　this　paper　presents　a　series　of　simulation　analysis　of　the　basic　operation　data　of　the　sub-critical　Rankin　cycle　with　several　typical　working　fluids.　The　results　show　that　there　is　an　optimal　evaporation　temperature　by　testing　output　net　power　of　the　system,　and　different　working　fluids　have　different　optimal　evaporation　temperature,　which　affects　significantly　output　net　power　of　the　system.　R290　is　selected　as　optimal　working　fluid　with　net　power　of　81.34　kW　in　the　optimal　evaporation　temperature　of　11.08°C　conditions.　The　results　can　be　the　basis　for　further　optimizing　the　cold　energy　power　generation　system.