In　the　condition　of　room　temperature　14.2~23.9°C,　ANAMMOX　bacteria　granule　sludge　is　inoculated　to　the　biofilter,　which　is　filled　with　lava　as　carrier.　In　the　inlet　sewage　of　initial　stages,　ρ(NH4+-N)=70　mg/L,　ρ(NO2--N)=90　mg/L,　the　removal　load　of　TN　is　0.12　kg/(m3·d).　After　105　days,　the　removal　load　of　TN　increases　to　2.22　kg/(m3·d).　The　quick　start-up　of　ANAMMOX　biofilter　was　realized　successfully.　In　the　start-up　process,　according　to　the　change　of　N2　yield,　apparent　color　of　biofilm,　nitrogen　removal　velocity　and　pH　value,　the　whole　start-up　process　can　be　divided　into　two　phases　including　domestication　and　quick　multiplication　of　ANAMMOX　bacteria.