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Mature landfill leachate with high ammonia concentration was treated by a combined system consisting of a up-flow anaerobic sludge blanket (UASB), an anoxic/aerobic (A/O) reactor and a sequencing batch reactor (SBR). Denitrification and methanogenesis were carried out simultaneously in the UASB and then the denitrification of nitrite and nitrate in the returned sludge were accomplished in the A/O reactor. The residual ammonia as well as nitrite and nitrate was removed in SBR. The partial biological nitrogen removal was achieved stably both in A/O reactor with FA control and in SBR with process control strategy with 94% and 93% nitrite accumulation, respectively, and microbial analysis data showed AOB and NOB, respectively, accounted for 3.5% and 0.2% of the total amount of bacteria in A/O reactor and accounted for 4% and 0.4% in SBR. when the total nitrogen (TN) concentration of influent leachate was about 2080 mg/L and the ammonia nitrogen concentration was about 1970 mg/L, Nitrification and nitrogen removal were usually both higher than 99% and the TN and NH4+-N of final effulent were about 12 mg/L and 6 mg/L, respectively. The key factor of achieving and stabilizing partial nitrification in combined system was the optimization of nitrifying communities in sludge owing to long-term application of FA inhibition and process control.
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