The　combination　of　chromatogram　and　chemical　analysis　was　adopted　to　optimize　the　methods　selected　to　measure　poly-P,　glycogen　and　PHA　intracellular　polymer　in　EBPR　system.　PHA　was　measured　by　GC　system,　glycogen　was　degraded　by　dilute　hydrochloric　acid　and　analyzed　by　glucose　commercial　kit,　and　poly-P　was　oxidized　by　potassium　persulfate　and　analyzed　by　molybdenum-antimony　anti-spectrophotometric　method.　Testing　of　the　proposed　method　for　PHA　analysis　shows　that　4%　sulfuric　acid　concentration　and　degradation　for　20　h　are　required　for　optimal　PHB　and　PHV　extraction,　while　0.6　mol/L　HCl　and　digestion　for　5　h　produce　the　highest　recovery　of　glycogen,　and　the　medical　glucose　kit　can　be　used　in　this　research.　Oxidation　for　30　min　at　120°C　is　recommended　for　the　recovery　of　poly-P.　The　results　of　precision　analysis　and　recovery　test　with　standard　addition　of　these　methods　show　that　they　possess　high　precision　and　are　convenient　for　laboratory　research　and　practical　application.