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In order to explore the optimal conditions of nitrifying sludge granulation, test was carried out in a sequencing batch reactor(SBR) at operation conditions: temperature 28°C, aeration 0.2 m3 · h-1, dissolved oxygen(DO) > 2.0 mg · L-1, sludge age(SRT) 15 d, leading to settling time shorten to 2 min. The granulation of nitrifying floccular activated sludge was achieved successfully after 80 stable cycles(19 d) by parameter control of ammonia oxidation process, by optimization of aerobic time and against over-aeration, and average diameter of sludge granular obtained was between 1.5-2.0 mm. The efficiency of COD and ammonia removal was 80% and 95%, respectively. The nitrite accumulation rate(NO2--N/NOx--N) was over 95%. The quantitative results from fluorescence in situ hybridization(FISH) analysis for molecular biology show that the dominant micro-organism was still AOB, about 17.8% of total bacteria, while NOB only 0.6%. The main factors for stable performance of nitrification were FA inhibition and real-time control. © All Rights Reserved.
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