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The shift of nitrifier population within a partial nitrification reactor was investigated using fluorescence in-situ hybridization (FISH) techniques and the floc morphology was observed using scan electron microscope (SEM). Partial nitrification to nitrite was achieved quickly by using real-time aeration duration control when domestic wastewater was treated in a lab-scale sequencing batch reactor (SBR). The average nitrite accumulation ratio was above 93%. The morphology photographs observed using SEM indicated that the sludge showed a shift towards spherical and small rod-shaped clusters from the diverse morphology. FISH analysis showed that ammonia-oxidizing bacteria (AOB) gradually out-competed nitrite-oxidizing bacteria (NOB). AOB population percentage increased from 3.5% (during the start-up period) to 8.5% (after achieving nitritation for 102 days), while NOB population percentage decreased from 3.1% to less than 0.5%. Accordingly, the long-term operation with the use of real-time aeration duration control was not only favorable for AOB community optimisation, but also for the achievement of partial nitrification in practice.
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