Assessment of partial nitrification achieved by real-time aeration duration control through microbial population shift using FISH and SEM - 文章详情页

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摘要：

The　shift　of　nitrifier　population　within　a　partial　nitrification　reactor　was　investigated　using　fluorescence　in-situ　hybridization　(FISH)　techniques　and　the　floc　morphology　was　observed　using　scan　electron　microscope　(SEM).　Partial　nitrification　to　nitrite　was　achieved　quickly　by　using　real-time　aeration　duration　control　when　domestic　wastewater　was　treated　in　a　lab-scale　sequencing　batch　reactor　(SBR).　The　average　nitrite　accumulation　ratio　was　above　93%.　The　morphology　photographs　observed　using　SEM　indicated　that　the　sludge　showed　a　shift　towards　spherical　and　small　rod-shaped　clusters　from　the　diverse　morphology.　FISH　analysis　showed　that　ammonia-oxidizing　bacteria　(AOB)　gradually　out-competed　nitrite-oxidizing　bacteria　(NOB).　AOB　population　percentage　increased　from　3.5%　(during　the　start-up　period)　to　8.5%　(after　achieving　nitritation　for　102　days),　while　NOB　population　percentage　decreased　from　3.1%　to　less　than　0.5%.　Accordingly,　the　long-term　operation　with　the　use　of　real-time　aeration　duration　control　was　not　only　favorable　for　AOB　community　optimisation,　but　also　for　the　achievement　of　partial　nitrification　in　practice.

关键词：

Ammonia Bacteria Batch reactors Electron microscopes Fluorescence Fluorescence microscopy Morphology Nitration Nitrification Real time control Wastewater treatment

作者机构：

[ 1 ] [Guo, Jian-Hua]School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China
[ 2 ] [Wang, Shu-Ying]Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100124, China
[ 3 ] [Zheng, Ya-Nan]Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100124, China
[ 4 ] [Peng, Yong-Zhen]School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China
[ 5 ] [Peng, Yong-Zhen]Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100124, China
[ 6 ] [Liu, Yang]China Water Holdings Pte. Ltd., Beijing 100020, China
[ 7 ] [Sun, Zhi-Rong]Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100124, China

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电子邮件地址：

pyz@bjut.edu.cn

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