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How to get stable and sufficient nitrite as an electronic acceptor of anammox bacteria is a bottleneck of nitrogen removal in treatment of municipal wastewater by anaerobic ammonium oxidation reaction processes. Therefore, denitrification processes was considered as an alternative to obtain stable nitrite. The nitrite accumulation during denitrification processes was studied in a bench-scale sequencing batch reactor with methanol as carbon source by controlling initial C/N(COD/NO3--N) ratios in influent. The results showed that, the shortage of methanol feed(C/N ratios 2--N and NO3--N goes up with the concentration of COD when C/N ratios
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