Renal　cell　carcinoma　is　one　of　the　most　common　urinary　system　tumors　in　adults,　it　is　usually　asymptomatic　in　its　early　stage　and　the　patients　are　often　diagnosed　late.　MicroRNA　has　a　higher　diagnostic　accuracy　than　traditional　markers　and　may　become　a　new　type　of　early　diagnostic　biomarker　for　kidney　cancer.　Three　computational　methods　and　several　bioinformatic　methods　including　PPI　network,　overall　survival　analysis　and　enrichment　analysis　were　used　to　identify　the　significant　differentially　expressed　miRNAs.　Thirteen　miRNAs　that　were　significantly　differentially　expressed　in　RCC　patients　were　identified,　10　of　them　have　been　proved　to　be　associated　with　kidney　cancer　in　other　studies,　miR-576,　miR-616　and　miR-133a-2　are　three　newly　discovered　biomarkers　of　RCC　in　this　study.　We　found　that　the　target　genes　of　miR-576　(CUL3　and　RAC1)　are　involved　in　the　regulation　of　multiple　cancer-related　biological　pathways,　and　the　target　gene　of　miR-616　(ASB13　and　FBXW2)　has　been　reported　to　be　associated　with　the　development　of　other　cancers.　Our　findings　may　have　guiding　significance　for　the　early　diagnosis　of　renal　cell　carcinoma.