In　this　paper,　the　cross-flow　trickling　biofilter　was　used　to　remove　toluene　from　an　artificial　waste　gas　in　order　to　resolve　problems　of　the　columned　trickling　biofilter,　such　as　low　effective　zone,　high　pressure　and　easily　clogging.　The　biofilter　was　inoculated　with　Pseudomonas　Pudida　and　packed　with　biological　ceramic　pellets.　The　start-up　process　and　the　performance　of　the　biofilter　under　different　residence　time　and　volumetric　loading　were　studied.　The　effects　of　the　temperature　of　nutrients　on　the　removal　capacity　of　the　biofilter　were　also　investigated.　7　days　were　needed　to　start　the　biofilter.　Complete　toluene　removal　could　be　achieved　when　the　residence　time　were　144　s,　72　s,　48　s　and　36　s　and　the　maximum　volumetric　removal　loading　of　276.62　gm-3·h-1　was　achieved.　The　optimum　temperature　of　nutrients　was　in　the　range　of　28C　to　34C.　The　results　indicate　that　the　cross-flow　trickling　biofilter　inoculated　with　Pseudomonas　Pudida　is　feasible　and　an　effective　treatment　process　to　treat　toluene　waste　gas.　The　special　structure　and　operation　of　the　cross-flow　trickling　biofilter　can　enhance　the　effective　zone　of　the　reactor　and　adjust　the　humidity　of　the　reactor　which　is　suitable　to　treat　large　volumes　of　volatile　organic　compounds.　The　change　of　residence　time　has　little　influence　on　the　removal　capacity　of　the　biofilter　which　can　be　recovered　quickly.　To　control　the　temperature　of　the　nutrients　we　can　adjust　the　environmental　temperature　of　the　microorganisms　which　could　improve　the　removal　efficiency　effectively.