According　to　the　growth　and　metabolism　characteristics　of　CM1　cell,　a　new　low-serum　medium　was　successfully　developed,　which　was　added　2%　new　calf　serum　(NCS)　in　the　base　of　medium.　In　comparison　with　the　culture　in　the　medium　with　10%　fetal　bovine　serum　(FBS),　the　morphology　was　the　same　and　the　growth　was　better　in　the　culture　with　2%　NCS.　A　maximum　cell　density,　24.2105/mL,　was　obtained　at　120　h　in　the　culture　with　4　mmol/L　glutamine,　and　increased　by　80.6%　than　that　in　the　culture　without　glutamine.　The　number　of　cells　in　S　phase　decreased　with　the　increase　of　initial　glutamine　concentration,　and　it　was　benefit　for　the　synthesis　of　DNA.　With　the　increase　of　initial　glutamine　concentration　from　0　mmol/L　to　4.0　mmol/L,　The　specific　consumption　rates　of　glucose　decreased　by　16.5%,　and　the　consumption　of　cell　to　lysine,　valine,　and　isoleucine　decreased　significantly.　The　key　metabolic　enzymes　activities　of　hexokinase,　pyruvate　kinase,　and　lactate　dehydrogenase　decreased　with　the　increase　of　glutamine,　showing　that　the　flux　of　glycolysis　was　decreased.　A　higher　activity　of　glutamate-oxalacetic　aminotransferase　showed　that　the　transamination　pathway　was　still　capital　in　the　metabolism　of　glutamine.