The correspondence study of BIAcore binding and anti-HBV effect in vitro by KA-01 and its main ingredient - 文章详情页

作者：

Ouyang, Yan-Ling (Ouyang, Yan-Ling.) | Li, Ze-Lin (Li, Ze-Lin.) | Ma, Hong-Tao (Ma, Hong-Tao.) | Liu, Ping (Liu, Ping.) | Zeng, Yi (Zeng, Yi.)

收录：

EI Scopus PKU CSCD

摘要：

In　order　to　discover　activated　ingredient　form　Chinese　herbal　medicine　and　to　bind　KA-01and　its　main　ingredients　to　HBsAg　and　HBeAg　protein　in　CM5　by　BIAcore　3000,　the　HepG2.2.15　cell　was　cultured　in　vitro　to　observe　the　inhibitory　effect　of　KA-01　and　its　main　ingredient.　The　inhibitory　effect　of　KA-01　and　its　main　ingredients　on　protein　secretion　of　HBV　were　tested　by　Laser　Confocal　Microscope.　The　results　indicated　that　the　value　of　KA-01and　its　main　ingredients　of　A,　B,　C,　D　binding　with　HBsAg　protein　were　200,　183.7,　162.1,　39.1,　66.6　RU　respectively,　but　almost　0　with　HBeAg　protein.　The　inhibitory　effect　of　KA-01　on　secretion　of　HBsAg　and　HBeAg　were　62.01%　and　30.59%　respectively.　The　inhibitory　effect　of　A　on　secretion　of　HBsAg　and　HBeAg　were　54.26%　and　14.15%　respectively.　The　inhibitory　effect　of　B　on　secretion　of　HBsAg　and　HBeAg　were　50.97%　and　27.92%　respectively.　The　inhibitory　effect　of　C　on　secretion　of　HBsAg　and　HBeAg　were　17.54%　and　8.06%　respectively.　The　conclusion　is　that　KA-01and　its　main　ingredients　of　A,　B　have　the　inhibitory　effect　on　HBV.

关键词：

Cell culture Medicine Plants (botany) Proteins

作者机构：

[ 1 ] [Ouyang, Yan-Ling]Laboratory of Viral Pharmacology, College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
[ 2 ] [Li, Ze-Lin]Laboratory of Viral Pharmacology, College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
[ 3 ] [Ma, Hong-Tao]Laboratory of Viral Pharmacology, College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
[ 4 ] [Liu, Ping]Laboratory of Viral Pharmacology, College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
[ 5 ] [Zeng, Yi]Laboratory of Viral Pharmacology, College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China

通讯作者信息：

电子邮件地址：

查看成果更多字段

成果类型
所属机构

所有年份指定年份从至