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Simultaneous biological phosphorus and nitrogen removal by enhanced anoxic phosphate uptake was investigated in an anaerobic-aerobic-anoxic-aerobic sequencing batch reactor (model II). The ratio of the anoxic phosphate uptake to the aerobic phosphate uptake capacity increased from 28.2% to 68.3% by insert an anoxic phase in an anaerobic-aerobic SBR (model I). The model II SBR system showed stable phosphorus and nitrogen removal performance. Average removal efficiencies of ρ(COD), ρ(PO3--P), and ρ(TN) were 92.0%, 98.0%, and 81.5%, respectively. Through batch experiment, it was found that ρ(NO2--N) (up to 30 mg/L) was not detrimental to the anoxic phosphate uptake and could serve as an electron acceptor like ρ(NO3--N). In fact, the phosphate uptake rate was even faster in the presence of ρ(NO2--N) as an electron acceptor compared to the presence of ρ(NO3--N). It was found that on-line sensor values of pH and EORP were somehow related with the dynamic behaviors of nutrient concentrations (ρ(COD), ρ(NH4+-N), ρ(NO3--N), and ρ(PO33--P)) in the SBR. The inflexion of EORP profile corresponding to the end of phosphorus released in anaerobic phase; the inflexion of EORP and pH profiles corresponding to the end of nitrification in aerobic phase I; the inflexion of EORP and pH profiles corresponding to the end of denitrification in anoxic phase; the inflexion of EORP and pH profiles corresponding to the end of ρ(COD) exhaust and phosphorus uptake in aerobic phase II. These pH and EORP profile characteristic point could be used as real-time control parameters to adjust the duration of each operational phase in the model II SBR, thus could enhance the nitrogen and phosphorus removal efficiency.
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