To　fully　analyze　the　reaction　mixtures　from　cyclohexane　oxidation,　a　united　analyzing　method　was　established　based　on　the　comparison　of　feasibility　of　all　kinds　of　analysis　methods,　including　analyzing　adipic　acid,　glutaric　acid,　and　succinic　acid　(abbreviation　＇three　acids＇)　by　HPLC　technique,　analyzing　cyclohexane,　cyclohexanol,　and　cyclohexanone　by　GC　technique,　and　analyzing　the　peroxide　and　ester　by　chemical　titration.　In　order　to　make　an　accurate　analysis　of　the　＇three　acids＇　in　the　reaction　mixtures　by　HPLC,　the　effects　of　several　factors　such　as　UV　wavelength,　mobile　phase　composition　and　its　pH　value,　and　mobile　phase　flow　rate,　on　the　retention　time　and　separation　efficiency　of　the　＇three　acids＇　were　investigated.　It　was　found　that　the　optimal　conditions　for　the　separation　of　the　＇three　acids＇　were:　The　UV　wavelength　was　212　nm,　the　composition　of　flow　phase　was　potassium　dihydrogen　phosphate-methanol　buffered　solution　with　15%-30%　methanol　and　its　pH　=　3-4,　and　the　flow　phase　velocity　was　0.8　mL/min.　In　addition,　the　precision　and　accuracy　of　the　above　three　analytical　methods　were　analyzed,　and　the　results　showed　that　the　relative　standard　deviations　of　the　three　analysis　methods　were　less　than　0.71%,　confirming　that　the　chemical　analysis　method　was　reliable.