A　high-frequency　and　micro-amplitude　vibration　based　micro-operation　manipulator　prototype　is　developed,　in　which　the　3-RUU　pure　translational　parallel　mechanism　is　adopted　as　the　micro-motion　mechanism,　and　a　high-frequency　and　micro-amplitude　vibration　unit　is　fixed　on　its　movable　platform,　the　operational　needle　connected　with　the　micro-vibration　unit　can　moves　with　the　movable　platform　in　a　larger　spatial　region,　meanwhile　the　reciprocative　piercing　motion　of　needle　is　achieved　by　the　actuation　of　micro-vibration　unit.　As　a　result,　the　integrative　motion　of　operational　needle　for　micro-dissection　operation　is　generated.　The　micro-operation　manipulator　prototype　is　designed　and　fabricated,　and　a　micro-operation　experimental　system　is　established　where　the　micro-manipulator　is　used　as　a　basic　module.　Performance　of　the　experimental　system　is　tested　through　initial　operations　of　cow　oocyte.　The　results　shows　that　the　developed　experimental　system　can　complete　proleptic　opertional　tasks　successfully,　and　both　the　elastic　distortion　and　injure　of　cow　oocyte　are　smaller.　The　presented　experimental　system　can　be　adopted　as　the　physical　platform　for　microscopical　operation　study.