Simultaneous　removal　of　hydrogen　sulfide　(H2S)　and　volatile　organic　sulfur　compounds　(VOSCs)　in　off-gas　mixture　from　a　wastewater　treatment　plant　(WWTP)　is　difficult　due　to　the　occasional　inhibitory　effects　of　H2S　on　VOSC　degradation.　In　this　study,　a　two-stage　bio-trickling　filter　(BTF)　system　was　developed　to　treat　off-gas　mixture　from　a　real　WWTP　facility.　At　an　empty　bed　retention　time　of　40　s,　removal　efficiencies　of　H2S,　methanethiol,　dimethyl　sulfide,　and　dimethyl　disulfide　were　90.1,　88.4,　85.8,　and　61.8%,　respectively.　Furthermore,　the　effect　of　lifting　load　shock　on　system　performance　was　investigated　and　results　indicated　that　removal　ofboth　H2S　and　VOSCs　was　slightly　affected.　Illumina　Miseq　sequencing　revealed　that　the　microbial　community　of　first-stage　BTF　contained　high　abundance　of　H2S-affinity　genera　including　Acidithiobacillus　(51.43%),　Metallibacterium　(25.35%),　and　Thionomas　(8.08%).　Analysis　of　mechanism　demonstrated　that　first　stage　of　BTF　removed　86.1%　of　H2S,　mitigating　the　suppression　on　VOSC　degradation　in　second　stage　of　BTF.　Overall,　the　two-stage　BTF　system,　an　innovative　bioprocess,　can　simultaneously　remove　H2S　and　VOSC.　(c)　Higher　Education　Press　and　Springer-Verlag　GmbH　Germany,　part　of　Springer　Nature　2019