A　high-frequency　and　micro-amplitude　vibration　based　manipulator　for　microscopical　dissection　is　proposed,　in　which　the　3-RUU　parallel　mechanism　having　three　pure　translational　degrees　is　adopted　as　the　micro-motion　mechanism,　and　a　PZT　unit　generating　high-frequency　and　micro-amplitude　vibration　is　fixed　on　movable　platform　of　the　micro-motion　mechanism.　Moving　along　with　the　large　motion　of　movable　platform　and　synchronously　actuated　by　the　high-frequency　and　micro-amplitude　vibration　of　the　PZT　unit,　the　operational　needle　can　perform　the　micro-dissection　tasks.　The　dimensional　model　of　the　3-RUU　micro-motion　mechanism　is　established,　the　performance　atlas　describing　the　relationships　between　the　dimensional　parameter　combinations　and　mechanism　performances　are　protracted,　and　the　kinematic　parameters　of　micro-motion　mechanism　are　determined　by　means　of　these　performance　atlas.　Using　the　finite　element　method,　the　static　and　dynamic　characteristics　of　the　micro-motion　mechanism　are　analyzed,　and　the　structural　parameters　of　the　micro-motion　mechanism　as　well　as　the　driving　unit　are　designed　ulteriorly.　According　to　the　obtained　kinematic　and　structural　parameters,　a　micro-dissection　manipulator　prototype　is　fabricated,　which　can　be　used　as　a　basic　module　in　a　microscopical　dissection　operational　experiment　system.