The　microfluidic　polymerase　chain　reaction　(PCR)　biochip　cycle　circuit　geometry　is　analyzed.　For　the　general　non-closed　loop　circuit　paradigm,　the　feasible　temperature　zone　arrangement　is　determined;　for　closed　loop　circuit　paradigm,　the　heat　exchange　efficiency　of　several　structural　forms　are　described.　Then　the　excimer　laser　etching　on　polymethyl　methacrylate　(PMMA)　plate　mechanism　is　studied,　with　the　etching　rate　curves　listing　by　experiments.　For　PCR　biochip　microchannel　fabrication,　the　channel　shape　and　the　bottom　surface　roughness　are　mainly　considered.　Mask　shape　is　designed　to　fit　with　the　channel　shape;　laser　polishing,　near　threshold　energy　etching　and　heat　annealing　for　stress　relief　are　adopted　to　promote　the　channel　surface　quality.　For　the　problem　of　chip　bonding,　the　hot　press　solution　is　utilized　at　last.　The　hot　press　bonding　system　is　constructed,　the　optimal　bonding　parameters　is　acquired　by　tests.　For　driving　and　controlling　of　the　micro　continuous　flow,　a　precise　pneumatic　syringe　pump　system　is　developed.　By　utilizing　the　PCR　biochip　and　pump　system,　an　experiment　is　performed　and　the　amplified　product　of　a　170　bp　DNA　template　chain　is　successfully　acquired.